4n7f

X-ray diffraction
1.1Å resolution

Crystal structure of 3rd WW domain of human Nedd4-1

Released:

Function and Biology Details

Reaction catalysed:
(1a) S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [HECT-type E3 ubiquitin transferase]-L-cysteine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + S-ubiquitinyl-[HECT-type E3 ubiquitin transferase]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E3 ubiquitin-protein ligase NEDD4 Chains: A, B
Molecule details ›
Chains: A, B
Length: 38 amino acids
Theoretical weight: 4.45 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P46934 (Residues: 841-874; Coverage: 3%)
Gene names: KIAA0093, NEDD4, NEDD4-1, PIG53
Sequence domains: WW domain

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-D
Spacegroup: P4122
Unit cell:
a: 68.411Å b: 68.411Å c: 37.248Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.181 0.2
Expression system: Escherichia coli BL21(DE3)