4n78

X-ray diffraction
2.43Å resolution

The WAVE Regulatory Complex Links Diverse Receptors to the Actin Cytoskeleton

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
Cytoplasmic FMR1-interacting protein 1 Chain: A
Molecule details ›
Chain: A
Length: 1253 amino acids
Theoretical weight: 145.36 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q7L576 (Residues: 1-1253; Coverage: 100%)
Gene names: CYFIP1, KIAA0068
Sequence domains:
Nck-associated protein 1 Chain: B
Molecule details ›
Chain: B
Length: 1128 amino acids
Theoretical weight: 128.94 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q9Y2A7 (Residues: 1-1128; Coverage: 100%)
Gene names: HEM2, KIAA0587, NAP1, NCKAP1
Sequence domains: Membrane-associated apoptosis protein
Wiskott-Aldrich syndrome protein family member 1 Chain: D
Molecule details ›
Chain: D
Length: 559 amino acids
Theoretical weight: 61.73 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q92558 (Residues: 1-559; Coverage: 100%)
Gene names: KIAA0269, SCAR1, WASF1, WAVE1
Sequence domains: WH2 motif
Structure domains:
Protein BRICK1 Chain: E
Molecule details ›
Chain: E
Length: 75 amino acids
Theoretical weight: 8.76 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8WUW1 (Residues: 1-75; Coverage: 100%)
Gene names: BRK1, C3orf10, HSPC300, MDS027
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces
Abl interactor 2 Chain: F
Molecule details ›
Chain: F
Length: 514 amino acids
Theoretical weight: 55.81 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9NYB9 (Residues: 1-513; Coverage: 100%)
Gene names: ABI2, ARGBPIA
Sequence domains:
WIRS Chain: P
Molecule details ›
Chain: P
Length: 15 amino acids
Theoretical weight: 1.69 KDa

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 97.492Å b: 114.75Å c: 323.605Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.189 0.189 0.209
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli