PDBe 4n0a

X-ray diffraction
3.15Å resolution

Crystal structure of Lsm2-3-Pat1C complex from Saccharomyces cerevisiae

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero 14-mer (preferred)
monomeric
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
U6 snRNA-associated Sm-like protein LSm2 Chains: C, D, G
Molecule details ›
Chains: C, D, G
Length: 109 amino acids
Theoretical weight: 12.8 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P38203 (Residues: 1-95; Coverage: 100%)
Gene names: LSM2, SMX5, SNP3, YBL026W, YBL0425
Sequence domains: LSM domain
U6 snRNA-associated Sm-like protein LSm3 Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 89 amino acids
Theoretical weight: 10.04 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P57743 (Residues: 1-89; Coverage: 100%)
Gene names: LSM3, SMX4, USS2, YLR438C-A
Sequence domains: LSM domain
Structure domains: SH3 type barrels.
DNA topoisomerase 2-associated protein PAT1 Chains: H, I, J
Molecule details ›
Chains: H, I, J
Length: 380 amino acids
Theoretical weight: 43.19 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P25644 (Residues: 422-796; Coverage: 47%)
Gene names: MRT1, PAT1, YCR077C, YCR77C

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-1
Spacegroup: C2
Unit cell:
a: 138.447Å b: 251.511Å c: 120.31Å
α: 90° β: 112.09° γ: 90°
R-values:
R R work R free
0.274 0.272 0.3
Expression system: Escherichia coli