› 4mtk

X-ray diffraction
3.32Å resolution

Crystal structure of PA0091 VgrG1, the central spike of the Type VI Secretion System

Released:
DOI: 10.2210/pdb4mtk/pdb
PDB entry 4mtk coloured by chain, front view.
PDB entry 4mtk coloured by chain, side view.
PDB entry 4mtk coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Sycheva LV, Shneider MM, Leiman PG

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191490 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Type VI secretion system spike protein VgrG1a Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 643 amino acids
Theoretical weight: 72.1 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9I741 (Residues: 1-643; Coverage: 100%)
Gene names: PA0091, vgrG1a
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand SO4 256 x SO4
Ligand TAM 2 x TAM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P61
Unit cell:
a: 168.275Å b: 168.275Å c: 652.849Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.182 0.17 0.195
Expression system: Escherichia coli

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