X-ray diffraction
3.7Å resolution

Structure of active human M2 muscarinic acetylcholine receptor bound to the agonist iperoxo and allosteric modulator LY2119620


Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Muscarinic acetylcholine receptor M2 Chain: A
Molecule details ›
Chain: A
Length: 351 amino acids
Theoretical weight: 39.95 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: P08172 (Residues: 1-18, 19-466; Coverage: 70%)
Gene name: CHRM2
Sequence domains: 7 transmembrane receptor (rhodopsin family)
Structure domains: Rhodopsin 7-helix transmembrane proteins
Nanobody 9-8 Chain: B
Molecule details ›
Chain: B
Length: 125 amino acids
Theoretical weight: 13.43 KDa
Source organism: Lama glama
Expression system: Escherichia coli BL21(DE3)
Structure domains: Immunoglobulins

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 59.032Å b: 77.399Å c: 163.822Å
α: 90° β: 90° γ: 90°
R R work R free
0.255 0.25 0.301
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli BL21(DE3)