PDBe 4mml

X-ray diffraction
1.8Å resolution

D40A Hfq from Pseudomonas aeruginosa

Released:

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo hexamer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
RNA-binding protein Hfq Chain: A
Molecule details ›
Chain: A
Length: 82 amino acids
Theoretical weight: 9.07 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9HUM0 (Residues: 1-82; Coverage: 100%)
Gene names: PA4944, hfq
Sequence domains: Hfq protein
Structure domains: SH3 type barrels.

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P6
Unit cell:
a: 65.79Å b: 65.79Å c: 28.13Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.172 0.164 0.203
Expression system: Escherichia coli BL21(DE3)