X-ray diffraction
2.1Å resolution

Crystal structure of the SpyTag/SpyCatcher complex


Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Fibronectin binding protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 116 amino acids
Theoretical weight: 12.42 KDa
Source organism: Streptococcus pyogenes
Expression system: Escherichia coli BL21(DE3)
  • Canonical: Q8G9G1 (Residues: 440-552; Coverage: 16%)
Gene names: E0F66_06355, fba2
Sequence domains:
Structure domains: Immunoglobulins
SpyTag Chains: B, D
Molecule details ›
Chains: B, D
Length: 13 amino acids
Theoretical weight: 1.48 KDa
Source organism: Streptococcus pyogenes
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P1
Unit cell:
a: 31.621Å b: 38.527Å c: 44.491Å
α: 83.7° β: 78.25° γ: 89.72°
R R work R free
0.2 0.198 0.225
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided