4mf2

X-ray diffraction
2.4Å resolution

Structure of human DNA polymerase beta complexed with O6MG as the template base in a 1-nucleotide gapped DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
3 distinct DNA molecules
Macromolecules (4 distinct):
DNA polymerase beta Chain: A
Molecule details ›
Chain: A
Length: 325 amino acids
Theoretical weight: 37.08 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P06746 (Residues: 11-335; Coverage: 97%)
Gene name: POLB
Sequence domains:
Structure domains:
synthetic template DNA Chain: T
Molecule details ›
Chain: T
Length: 16 nucleotides
Theoretical weight: 4.86 KDa
Source organism: Homo sapiens
Expression system: Not provided
synthetic upstream primer Chain: P
Molecule details ›
Chain: P
Length: 10 nucleotides
Theoretical weight: 3.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
synthetic downstream primer Chain: D
Molecule details ›
Chain: D
Length: 5 nucleotides
Theoretical weight: 1.54 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P21
Unit cell:
a: 54.438Å b: 79.265Å c: 54.789Å
α: 90° β: 105.66° γ: 90°
R-values:
R R work R free
0.202 0.198 0.279
Expression systems:
  • Escherichia coli
  • Not provided