4md9

X-ray diffraction
3.5Å resolution

Crystal Structure of symmetric CK2 holoenzyme with mutated alpha subunit (F121E truncated at aa 336)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Casein kinase II subunit beta Chains: A, B, C, D, I, J, N, O
Molecule details ›
Chains: A, B, C, D, I, J, N, O
Length: 215 amino acids
Theoretical weight: 24.97 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P67870 (Residues: 1-215; Coverage: 100%)
Gene names: CK2N, CSNK2B, G5A
Sequence domains: Casein kinase II regulatory subunit
Structure domains:
Casein kinase II subunit alpha Chains: E, F, G, H, K, L, M, P
Molecule details ›
Chains: E, F, G, H, K, L, M, P
Length: 336 amino acids
Theoretical weight: 40.14 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P68400 (Residues: 1-336; Coverage: 86%)
Gene names: CK2A1, CSNK2A1
Sequence domains: Protein kinase domain
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: C2
Unit cell:
a: 283.924Å b: 115.707Å c: 208.223Å
α: 90° β: 119.22° γ: 90°
R-values:
R R work R free
0.23 0.229 0.259
Expression system: Escherichia coli BL21