4m42

X-ray diffraction
2.04Å resolution

RB69 DNA polymerase ternary complex with dG/dT at position n-4 of primer/tempLate duplex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
DNA-directed DNA polymerase Chain: A
Molecule details ›
Chain: A
Length: 903 amino acids
Theoretical weight: 104.45 KDa
Source organism: Enterobacteria phage RB69
Expression system: Escherichia coli
UniProt:
  • Canonical: Q38087 (Residues: 1-903; Coverage: 100%)
Gene name: 43
Sequence domains:
Structure domains:
DNA template Chain: T
Molecule details ›
Chain: T
Length: 17 nucleotides
Theoretical weight: 5.2 KDa
Source organism: Escherichia phage RB69
Expression system: Not provided
DNA primer Chain: P
Molecule details ›
Chain: P
Length: 13 nucleotides
Theoretical weight: 3.99 KDa
Source organism: Escherichia phage RB69
Expression system: Not provided

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: P212121
Unit cell:
a: 78.538Å b: 118.657Å c: 130.651Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.187 0.185 0.224
Expression systems:
  • Escherichia coli
  • Not provided