Function and Biology Details
Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).
TSAVLQ-|-SGFRK-NH(2) and SGVTFQ-|-GKFKK the two peptides corresponding to the two self-cleavage sites of the SARS 3C-like proteinase are the two most reactive peptide substrates. The enzyme exhibits a strong preference for substrates containing Gln at P1 position and Leu at P2 position.
- not assigned
Structure analysis Details
Length: 327 amino acids
Theoretical weight: 36.86 KDa
Source organism: Severe acute respiratory syndrome-related coronavirus
Expression system: Escherichia coli
- Canonical: P0C6U8 (Residues: 1541-1858; Coverage: 7%)
Length: 76 amino acids
Theoretical weight: 8.58 KDa
Source organism: Bos taurus
- Canonical: P62992 (Residues: 1-76; Coverage: 49%)
Sequence domains: Ubiquitin family
Structure domains: Phosphatidylinositol 3-kinase Catalytic Subunit; Chain A, domain 1