PDBe 4lqs

X-ray diffraction
3.3Å resolution

Crystal structure of the Cbk1-Mob2 kinase-coactivator complex

Released:

Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Serine/threonine-protein kinase CBK1 Chain: A
Molecule details ›
Chain: A
Length: 508 amino acids
Theoretical weight: 58.76 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P53894 (Residues: 251-756; Coverage: 67%)
Gene names: CBK1, N1727, YNL161W
Sequence domains: Protein kinase domain
CBK1 kinase activator protein MOB2 Chain: B
Molecule details ›
Chain: B
Length: 244 amino acids
Theoretical weight: 28.42 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli
UniProt:
  • Canonical: P43563 (Residues: 46-287; Coverage: 84%)
Gene names: MOB2, YFL034C-B, YFL035C, YFL035C-A
Sequence domains: Mob1/phocein family
Structure domains: MOB kinase activator

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06DA
Spacegroup: C2
Unit cell:
a: 138.43Å b: 79.98Å c: 117.58Å
α: 90° β: 117.6° γ: 90°
R-values:
R R work R free
0.272 0.27 0.307
Expression system: Escherichia coli