› 4lpa

X-ray diffraction
2.9Å resolution

Crystal structure of a Cdc6 phosphopeptide in complex with Cks1

Released:
DOI: 10.2210/pdb4lpa/pdb
PDB entry 4lpa coloured by chain, front view.
PDB entry 4lpa coloured by chain, side view.
PDB entry 4lpa coloured by chain, top view.
Source organism: Saccharomyces cerevisiae S288C
Primary publication:
Cks confers specificity to phosphorylation-dependent CDK signaling pathways.
McGrath DA, Balog ER, Kõivomägi M, Lucena R, Mai MV, Hirschi A, Kellogg DR, Loog M, Rubin SM
Nat Struct Mol Biol 20 1407-14 (2013)
PMID: 24186063

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-148993 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Cyclin-dependent kinases regulatory subunit Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 119 amino acids
Theoretical weight: 14.26 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P20486 (Residues: 1-113; Coverage: 75%)
Gene names: CKS1, YBR1011, YBR135W
Sequence domains: Cyclin-dependent kinase regulatory subunit
Structure domains: Cyclin-dependent kinase, regulatory subunit

Ligands and Environments

No bound ligands
1 modified residue:
Ligand TPO 4 x TPO

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P41212
Unit cell:
a: 84.69Å b: 84.69Å c: 239.93Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.248 0.245 0.281
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

8 review citations

Ultrasensitivity part II: multisite phosphorylation, stoichiometric inhibitors, and positive feedback.
Ferrell et al. (2014)
7 more

4 mentions without citation

Physicochemical mechanisms of protein regulation by phosphorylation.
Nishi et al. (2014)
3 more