PDBe 4lnc

Neutron Diffraction
2.19Å resolution

Neutron structure of the cyclic glucose bound Xylose Isomerase E186Q mutant

Released:
Source organism: Streptomyces rubiginosus
Primary publication:
Neutron structure of the cyclic glucose-bound xylose isomerase E186Q mutant.
Acta Crystallogr. D Biol. Crystallogr. 70 414-20 (2014)
PMID: 24531475

Function and Biology Details

Reaction catalysed:
D-xylopyranose = D-xylulose
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Xylose isomerase Chain: A
Molecule details ›
Chain: A
Length: 388 amino acids
Theoretical weight: 43.2 KDa
Source organism: Streptomyces rubiginosus
Expression system: Escherichia coli
UniProt:
  • Canonical: P24300 (Residues: 1-388; Coverage: 100%)
Gene name: xylA
Sequence domains: Xylose isomerase-like TIM barrel
Structure domains: Divalent-metal-dependent TIM barrel enzymes

Ligands and Environments


No modified residues

Experiments and Validation Details

Spacegroup: I222
Unit cell:
a: 93.97Å b: 99.52Å c: 102.92Å α: 90° β: 90° γ: 90°
R-values:
R R work R free 0.288 0.284 0.322
Expression system: Escherichia coli