PDBe 4lk0

X-ray diffraction
3.91Å resolution

Crystal Structure Analysis of the E.coli holoenzyme/T7 Gp2 complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero heptamer (preferred)
Entry contents:
6 distinct polypeptide molecules
Macromolecules (6 distinct):
DNA-directed RNA polymerase subunit alpha Chains: A, B, G, H
Molecule details ›
Chains: A, B, G, H
Length: 239 amino acids
Theoretical weight: 26.46 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
Structure domains:
DNA-directed RNA polymerase subunit beta Chains: C, I
Molecule details ›
Chains: C, I
Length: 1342 amino acids
Theoretical weight: 150.82 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
DNA-directed RNA polymerase subunit beta' Chains: D, J
Molecule details ›
Chains: D, J
Length: 1407 amino acids
Theoretical weight: 155.37 KDa
Source organism: Escherichia coli BW2952
Expression system: Escherichia coli
DNA-directed RNA polymerase subunit omega Chains: E, K
Molecule details ›
Chains: E, K
Length: 91 amino acids
Theoretical weight: 10.25 KDa
Source organism: Escherichia coli DH1
Expression system: Escherichia coli
Structure domains: Eukaryotic RPB6 RNA polymerase subunit
RNA polymerase sigma factor RpoD Chains: F, L
Molecule details ›
Chains: F, L
Length: 522 amino acids
Theoretical weight: 60.32 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P00579 (Residues: 92-613; Coverage: 85%)
Gene names: JW3039, alt, b3067, rpoD
Sequence domains:
Structure domains:
Bacterial RNA polymerase inhibitor Chains: M, N
Molecule details ›
Chains: M, N
Length: 64 amino acids
Theoretical weight: 7.18 KDa
Source organism: Enterobacteria phage T7
Expression system: Escherichia coli
UniProt:
  • Canonical: P03704 (Residues: 1-64; Coverage: 100%)
Gene name: 2
Sequence domains: RNA polymerase inhibitor
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 186.364Å b: 206.283Å c: 307.999Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.221 0.219 0.26
Expression system: Escherichia coli