PDBe 4lck

X-ray diffraction
3.2Å resolution

Co-crystal structure of a T-box riboswitch stem I domain in complex with its cognate tRNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct RNA molecules
Macromolecules (3 distinct):
Ribosome-associated protein L7Ae-like Chains: A, D
Molecule details ›
Chains: A, D
Length: 81 amino acids
Theoretical weight: 8.44 KDa
Source organism: Bacillus subtilis subsp. subtilis str. 168
Expression system: Escherichia coli
UniProt:
  • Canonical: P46350 (Residues: 2-82; Coverage: 99%)
Gene names: BSU01090, rplGB, ybaB, ybxF
Sequence domains: Ribosomal protein L7Ae/L30e/S12e/Gadd45 family
Structure domains: 60s Ribosomal Protein L30; Chain: A;
tRNA-Gly Chains: B, E
Molecule details ›
Chains: B, E
Length: 75 nucleotides
Theoretical weight: 24.26 KDa
Source organism: Oceanobacillus iheyensis
Expression system: Not provided
Sequence domains: tRNA
T-BOX RIBOSWITCH STEM I Chains: C, F
Molecule details ›
Chains: C, F
Length: 102 nucleotides
Theoretical weight: 33.11 KDa
Source organism: Oceanobacillus iheyensis
Expression system: Not provided

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-E
Spacegroup: C2221
Unit cell:
a: 100.382Å b: 108.474Å c: 266.886Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.251
Expression systems:
  • Escherichia coli
  • Not provided