› 4l9l

X-ray diffraction
3.4Å resolution

Crystal structure of a human Valpha7.2/Vbeta13.2 MAIT TCR in complex with bovine MR1

Released:
DOI: 10.2210/pdb4l9l/pdb
PDB 4l9l coloured by chain and viewed from the front.
PDB 4l9l coloured by chain and viewed from the side.
PDB 4l9l coloured by chain and viewed from the top.
Source organisms:
Primary publication:
MAIT recognition of a stimulatory bacterial antigen bound to MR1.
López-Sagaseta J, Dulberger CL, McFedries A, Cushman M, Saghatelian A, Adams EJ
J Immunol 191 5268-77 (2013)
PMID: 24108697

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Beta-2-microglobulin; Major histocompatibility complex class I-related gene protein Chain: C
Molecule details ›
Chain: C
Length: 392 amino acids
Theoretical weight: 45.42 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: C1ITJ8 (Residues: 19-295; Coverage: 87%)
  • Canonical: P01888 (Residues: 21-118; Coverage: 100%)
Gene names: B2M, MR1
Sequence domains:
Structure domains:
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 208 amino acids
Theoretical weight: 23.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6P4G7 (Residues: 5-213; Coverage: 79%)
Gene name: TRA@
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chain: B
Molecule details ›
Chain: B
Length: 252 amino acids
Theoretical weight: 28.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand SO4 2 x SO4
1 modified residue:
Ligand KFP 1 x KFP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-B
Spacegroup: P212121
Unit cell:
a: 82.767Å b: 86.98Å c: 156.345Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.257 0.254 0.312
Expression systems:
  • Trichoplusia ni
  • Escherichia coli

Quick links

Citations

26 review citations

Engineering soluble T-cell receptors for therapy.
Robinson et al. (2021)
25 more

1 mention without citation

MAIT recognition of a stimulatory bacterial antigen bound to MR1.
López-Sagaseta et al. (2013)