› 4l4t

X-ray diffraction
2Å resolution

Structure of human MAIT TCR in complex with human MR1-6-FP

Released:
DOI: 10.2210/pdb4l4t/pdb
PDB entry 4l4t coloured by chain, front view.
PDB entry 4l4t coloured by chain, side view.
PDB entry 4l4t coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Recognition of vitamin B metabolites by mucosal-associated invariant T cells.
Patel O, Kjer-Nielsen L, Le Nours J, Eckle SB, Birkinshaw R, Beddoe T, Corbett AJ, Liu L, Miles JJ, Meehan B, Reantragoon R, Sandoval-Romero ML, Sullivan LC, Brooks AG, Chen Z, Fairlie DP, McCluskey J, Rossjohn J
Nat Commun 4 2142 (2013)
PMID: 23846752

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-208606 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Major histocompatibility complex class I-related gene protein Chains: A, C
Molecule details ›
Chains: A, C
Length: 271 amino acids
Theoretical weight: 31.71 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q95460 (Residues: 23-292; Coverage: 85%)
Gene name: MR1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, F
Molecule details ›
Chains: B, F
Length: 99 amino acids
Theoretical weight: 11.75 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
MAIT T-cell receptor alpha chain Chains: D, G
Molecule details ›
Chains: D, G
Length: 203 amino acids
Theoretical weight: 22.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chains: E, H
Molecule details ›
Chains: E, H
Length: 245 amino acids
Theoretical weight: 27.55 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins

Ligands and Environments

1 bound ligand:
Ligand 6FP 2 x 6FP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Spacegroup: C2
Unit cell:
a: 217.228Å b: 69.555Å c: 142.892Å
α: 90° β: 104.42° γ: 90°
R-values:
R R work R free
0.186 0.184 0.225
Expression system: Escherichia coli BL21

Quick links

Citations

88 review citations

Regional specialization within the intestinal immune system.
Mowat et al. (2014)
87 more

9 mentions without citation

Biased MAIT TCR Usage Poised for Limited Antigen Diversity?
Souter et al. (2020)
8 more