X-ray diffraction
1.8Å resolution

Crystal structure of a putative transcriptional regulator from Saccharomonospora viridis in complex with choline

Entry authors: Filippova EV, Minasov G, Shuvalova L, Kiryukhina O, Endres M, Joachimiak A, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
HTH tetR-type domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 195 amino acids
Theoretical weight: 22.9 KDa
Source organism: Saccharomonospora viridis DSM 43017
Expression system: Escherichia coli BL21(DE3)
  • Canonical: C7MT25 (Residues: 93-287; Coverage: 68%)
Gene name: Svir_16270
Sequence domains:
Structure domains: Tetracycline Repressor, domain 2

Ligands and Environments

3 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 21-ID-G
Spacegroup: P212121
Unit cell:
a: 42.901Å b: 77.355Å c: 109.83Å
α: 90° β: 90° γ: 90°
R R work R free
0.202 0.199 0.259
Expression system: Escherichia coli BL21(DE3)