4ku0

X-ray diffraction
1.15Å resolution

Enterobacteria phage T4 gp5.4 PAAR repeat protein in complex with T4 gp5 beta-helix fragment

Released:
Source organism: Escherichia virus T4
Entry authors: Buth SA, Leiman PG, Shneider MM

Function and Biology Details

Reaction catalysed:
Hydrolysis of (1->4)-beta-linkages between N-acetylmuramic acid and N-acetyl-D-glucosamine residues in a peptidoglycan and between N-acetyl-D-glucosamine residues in chitodextrins
Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Gp5C Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 96 amino acids
Theoretical weight: 9.86 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P16009 (Residues: 484-575; Coverage: 16%)
Gene name: 5
Sequence domains: Gp5 C-terminal repeat (3 copies)
Baseplate puncturing device gp5.4 Chain: D
Molecule details ›
Chain: D
Length: 96 amino acids
Theoretical weight: 10.1 KDa
Source organism: Escherichia virus T4
Expression system: Escherichia coli
UniProt:
  • Canonical: P39234 (Residues: 2-97; Coverage: 99%)
Gene names: 5.4, y08B
Sequence domains: PAAR motif
Structure domains: Tumour Suppressor Smad4

Ligands and Environments


No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P21
Unit cell:
a: 46.302Å b: 49.327Å c: 84.057Å
α: 90° β: 96.19° γ: 90°
R-values:
R R work R free
0.137 not available 0.171
Expression system: Escherichia coli