PDBe 4krc

X-ray diffraction
2.6Å resolution

Crystal Structure of Pho85-Pcl10-ATP-gamma-S Complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Cyclin-dependent protein kinase PHO85 Chain: A
Molecule details ›
Chain: A
Length: 317 amino acids
Theoretical weight: 36.36 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P17157 (Residues: 1-305; Coverage: 100%)
Gene names: P7102.18A, PHO85, SSG3, YPL031C
Sequence domains: Protein kinase domain
Structure domains:
PHO85 cyclin-10 Chain: B
Molecule details ›
Chain: B
Length: 207 amino acids
Theoretical weight: 23.75 KDa
Source organism: Saccharomyces cerevisiae S288C
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P53124 (Residues: 227-433; Coverage: 48%)
Gene names: G2838, PCL10, YGL134W
Sequence domains: Cyclin
Structure domains: Cyclin-like

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 50.145Å b: 65.378Å c: 80.254Å
α: 90° β: 100.61° γ: 90°
R-values:
R R work R free
0.207 0.204 0.27
Expression system: Escherichia coli BL21(DE3)