› 4ko9

X-ray diffraction
2.05Å resolution

Investigating the functional significance of the interlocked pair structural determinants in Pseudomonas aeruginosa azurin (V95I/Y108F)

Released:
DOI: 10.2210/pdb4ko9/pdb
PDB entry 4ko9 coloured by chain, front view.
PDB entry 4ko9 coloured by chain, side view.
PDB entry 4ko9 coloured by chain, top view.
Source organism: Pseudomonas aeruginosa PAO1
Entry authors: Inampudi KK, Tobin PH, Wilson CJ

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132372 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Azurin Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 128 amino acids
Theoretical weight: 13.96 KDa
Source organism: Pseudomonas aeruginosa PAO1
Expression system: Escherichia coli
UniProt:
  • Canonical: P00282 (Residues: 21-148; Coverage: 100%)
Gene names: PA4922, azu
Sequence domains: Copper binding proteins, plastocyanin/azurin family
Structure domains: Cupredoxins - blue copper proteins

Ligands and Environments

1 bound ligand:
Ligand CU 8 x CU
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P212121
Unit cell:
a: 52.682Å b: 97.054Å c: 100.276Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.188 0.226
Expression system: Escherichia coli

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