4kit

X-ray diffraction
3.6Å resolution

Crystal structure of human Brr2 in complex with the Prp8 Jab1/MPN domain

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
U5 small nuclear ribonucleoprotein 200 kDa helicase Chain: B
Molecule details ›
Chain: B
Length: 1739 amino acids
Theoretical weight: 198.79 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O75643 (Residues: 395-2129; Coverage: 81%)
Gene names: ASCC3L1, HELIC2, KIAA0788, SNRNP200
Sequence domains:
Structure domains:
Pre-mRNA-processing-splicing factor 8 Chain: C
Molecule details ›
Chain: C
Length: 278 amino acids
Theoretical weight: 31.86 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q6P2Q9 (Residues: 2064-2335; Coverage: 12%)
Gene names: PRPC8, PRPF8
Sequence domains:
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

2 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: P42212
Unit cell:
a: 240.002Å b: 240.002Å c: 201.089Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.199 0.197 0.235
Expression system: Spodoptera frugiperda