› 4kej

X-ray diffraction
2.55Å resolution

Crystal structure of mouse Ryanodine Receptor 2 (1-217) disease mutant R169Q

Released:
DOI: 10.2210/pdb4kej/pdb
PDB entry 4kej coloured by chain, front view.
PDB entry 4kej coloured by chain, side view.
PDB entry 4kej coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
Type 2 ryanodine receptor domain A contains a unique and dynamic α-helix that transitions to a β-strand in a mutant linked with a heritable cardiomyopathy.
Amador FJ, Kimlicka L, Stathopulos PB, Gasmi-Seabrook GM, Maclennan DH, Van Petegem F, Ikura M
J Mol Biol 425 4034-46 (2013)
PMID: 23978697

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-123214 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ryanodine receptor 2 Chain: A
Molecule details ›
Chain: A
Length: 217 amino acids
Theoretical weight: 24.14 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: E9Q401 (Residues: 1-217; Coverage: 4%)
Gene name: Ryr2
Sequence domains: Inositol 1,4,5-trisphosphate/ryanodine receptor
Structure domains: Trefoil (Acidic Fibroblast Growth Factor, subunit A)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: I41
Unit cell:
a: 112.859Å b: 112.859Å c: 38.094Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.238 0.236 0.297
Expression system: Escherichia coli BL21

Quick links

Citations

4 review citations

Ryanodine receptors: allosteric ion channel giants.
Van Petegem F. (2015)
3 more

1 mention without citation

Structural Insight Into Ryanodine Receptor Channelopathies.
Hadiatullah et al. (2022)