4jeh

X-ray diffraction
2.5Å resolution

Crystal Structure of Munc18a and Syntaxin1 lacking N-peptide complex

Released:

Function and Biology Details

Structure analysis Details

Assemblies composition:
hetero dimer (preferred)
hetero tetramer
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Syntaxin-binding protein 1 Chain: A
Molecule details ›
Chain: A
Length: 608 amino acids
Theoretical weight: 69.29 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P61765 (Residues: 1-594; Coverage: 100%)
Gene names: Stxbp1, Unc18a
Sequence domains: Sec1 family
Structure domains:
Syntaxin-1A Chain: B
Molecule details ›
Chain: B
Length: 243 amino acids
Theoretical weight: 28.31 KDa
Source organism: Rattus norvegicus
Expression system: Escherichia coli
UniProt:
  • Canonical: P32851 (Residues: 24-266; Coverage: 84%)
Gene names: Sap, Stx1a
Sequence domains: Syntaxin
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: I422
Unit cell:
a: 154.906Å b: 154.906Å c: 150.324Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.255 0.184 0.223
Expression system: Escherichia coli