› 4j4q

X-ray diffraction
2.65Å resolution

Crystal structure of active conformation of GPCR opsin stabilized by octylglucoside

Released:
DOI: 10.2210/pdb4j4q/pdb
PDB entry 4j4q coloured by chain, front view.
PDB entry 4j4q coloured by chain, side view.
PDB entry 4j4q coloured by chain, top view.
Source organism: Bos taurus
Primary publication:
Opsin, a structural model for olfactory receptors?
Park JH, Morizumi T, Li Y, Hong JE, Pai EF, Hofmann KP, Choe HW, Ernst OP
Angew Chem Int Ed Engl 52 11021-4 (2013)
PMID: 24038729

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-136066 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (4 distinct):
Rhodopsin Chain: A
Molecule details ›
Chain: A
Length: 348 amino acids
Theoretical weight: 39.03 KDa
Source organism: Bos taurus
UniProt:
  • Canonical: P02699 (Residues: 1-348; Coverage: 100%)
Gene name: RHO
Sequence domains:
Structure domains: Rhodopsin 7-helix transmembrane proteins
Guanine nucleotide-binding protein G(t) subunit alpha-1 Chain: B
Molecule details ›
Chain: B
Length: 11 amino acids
Theoretical weight: 1.26 KDa
Source organism: Bos taurus
Expression system: Not provided
UniProt:
  • Canonical: P04695 (Residues: 340-350; Coverage: 3%)
Gene name: GNAT1

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG, BMA, MAN
Carbohydrate polymer
Carbohydrate polymer : NEW Components: GLC
Carbohydrate polymer
4 bound ligands:
Ligand BOG 4 x BOG
Ligand PLM 1 x PLM
Ligand SO4 2 x SO4
Ligand ACT 1 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.2
Spacegroup: R32
Unit cell:
a: 241.265Å b: 241.265Å c: 109.561Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.217 0.23
Expression system: Not provided

Quick links

Citations

10 review citations

Constitutively active rhodopsin and retinal disease.
Park PS. (2014)
9 more

22 mentions without citation

Microbial and animal rhodopsins: structures, functions, and molecular mechanisms.
Ernst et al. (2014)
21 more