4j3o

X-ray diffraction
3.8Å resolution

Crystal structure of the FimD usher traversed by the pilus tip complex assembly composed of FimC:FimF:FimG:FimH

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
5 distinct polypeptide molecules
Macromolecules (5 distinct):
Protein FimG Chain: G
Molecule details ›
Chain: G
Length: 144 amino acids
Theoretical weight: 14.86 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P08190 (Residues: 24-167; Coverage: 100%)
Gene names: JW4282, b4319, fimG
Structure domains: Fimbrial-type adhesion domain
Type 1 fimbrin D-mannose specific adhesin Chain: H
Molecule details ›
Chain: H
Length: 279 amino acids
Theoretical weight: 29.08 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P08191 (Residues: 22-300; Coverage: 100%)
Gene names: JW4283, b4320, fimH
Sequence domains:
Structure domains: Fimbrial-type adhesion domain
Chaperone protein FimC Chain: C
Molecule details ›
Chain: C
Length: 211 amino acids
Theoretical weight: 23.58 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P31697 (Residues: 37-241; Coverage: 100%)
Gene names: JW4279, b4316, fimC
Sequence domains:
Structure domains: Immunoglobulins
Protein FimF Chain: F
Molecule details ›
Chain: F
Length: 154 amino acids
Theoretical weight: 16.18 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P08189 (Residues: 23-176; Coverage: 99%)
Gene names: JW4281, b4318, fimF
Sequence domains: Fimbrial protein
Structure domains: Fimbrial-type adhesion domain
Outer membrane usher protein FimD Chain: D
Molecule details ›
Chain: D
Length: 843 amino acids
Theoretical weight: 92.64 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P30130 (Residues: 46-878; Coverage: 100%)
Gene names: JW5780, b4317, fimD
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P42212
Unit cell:
a: 122.36Å b: 122.36Å c: 328.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.25 0.248 0.299
Expression system: Escherichia coli