4ivz

X-ray diffraction
3.1Å resolution

A Y37F mutant of C.Esp1396I bound to its highest affinity operator site OM

Released:

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
HTH cro/C1-type domain-containing protein Chains: A, B, E, F
Molecule details ›
Chains: A, B, E, F
Length: 82 amino acids
Theoretical weight: 9.51 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8GGH0 (Residues: 1-79; Coverage: 100%)
Gene name: esp1396IC
Sequence domains: Helix-turn-helix
Structure domains: lambda repressor-like DNA-binding domains
DNA (5'-D(*AP*TP*GP*TP*AP*GP*AP*CP*TP*AP*TP*AP*GP*TP*CP*GP*AP*CP*A)-3') Chains: C, G
Molecule details ›
Chains: C, G
Length: 19 nucleotides
Theoretical weight: 5.85 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Not provided
DNA (5'-D(*TP*TP*GP*TP*CP*GP*AP*CP*TP*AP*TP*AP*GP*TP*CP*TP*AP*CP*A)-3') Chains: D, H
Molecule details ›
Chains: D, H
Length: 19 nucleotides
Theoretical weight: 5.79 KDa
Source organism: Enterobacter sp. RFL1396
Expression system: Not provided

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 47.54Å b: 146.7Å c: 47.79Å
α: 90° β: 93.21° γ: 90°
R-values:
R R work R free
0.172 0.17 0.215
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided