X-ray diffraction
3.27Å resolution

Crystal structure of truncated Bovine viral diarrhea virus 1 E2 envelope protein

Primary publication:
Crystal structure of glycoprotein E2 from bovine viral diarrhea virus.
Proc Natl Acad Sci U S A 110 6805-10 (2013)
PMID: 23569276

Function and Biology Details

Reactions catalysed:
Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1)
Leu is conserved at position P1 for all four cleavage sites. Alanine is found at position P1' of the NS4A-NS4B cleavage site, whereas serine is found at position P1' of the NS3-NS4A, NS4B-NS5A and NS5A-NS5B cleavage sites
NTP + H(2)O = NDP + phosphate
ATP + H(2)O = ADP + phosphate
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecules (2 distinct):
Envelope glycoprotein E2 Chains: A, B
Molecule details ›
Chains: A, B
Length: 253 amino acids
Theoretical weight: 28.72 KDa
Source organism: Bovine viral diarrhea virus 1-NADL
Expression system: Trichoplusia ni
  • Canonical: P19711 (Residues: 781-1030; Coverage: 6%)
Sequence domains: Pestivirus envelope glycoprotein E2
Structure domains:

Ligands and Environments

Carbohydrate polymer : NEW Components: NAG
2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X25
Spacegroup: C2
Unit cell:
a: 136.72Å b: 54.45Å c: 95.92Å
α: 90° β: 92.23° γ: 90°
R R work R free
0.248 0.246 0.289
Expression system: Trichoplusia ni