4ik8

X-ray diffraction
1.55Å resolution

High resolution structure of GCaMP3 dimer form 1 at pH 7.5

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Green fluorescent protein; RCaMP Chain: A
Molecule details ›
Chain: A
Length: 448 amino acids
Theoretical weight: 50.54 KDa
Source organisms: Expression system: Escherichia coli
UniProt:
  • Canonical: K4DIE3 (Residues: 1-48, 51-59, 60-94, 95-127, 128-147, 279-283, 306-338, 342-354, 361-362, 368-432; Coverage: 58%)
  • Canonical: P42212 (Residues: 2-141, 145-170, 185-187, 201-206, 209-213; Coverage: 74%)
Gene name: GFP
Sequence domains: Green fluorescent protein
Structure domains: Green fluorescent protein

Ligands and Environments

1 bound ligand:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 126.34Å b: 47.352Å c: 66.74Å
α: 90° β: 100.27° γ: 90°
R-values:
R R work R free
0.189 0.179 0.209
Expression system: Escherichia coli