› 4iiq

X-ray diffraction
2.86Å resolution

Crystal structure of a human MAIT TCR in complex with bovine MR1

Released:
DOI: 10.2210/pdb4iiq/pdb
PDB 4iiq coloured by chain and viewed from the front.
PDB 4iiq coloured by chain and viewed from the side.
PDB 4iiq coloured by chain and viewed from the top.
Source organisms:
Primary publication:
The molecular basis for Mucosal-Associated Invariant T cell recognition of MR1 proteins.
López-Sagaseta J, Dulberger CL, Crooks JE, Parks CD, Luoma AM, McFedries A, Van Rhijn I, Saghatelian A, Adams EJ
Proc. Natl. Acad. Sci. U.S.A. 110 E1771-8 (2013)
PMID: 23613577

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Ig-like domain-containing protein Chain: A
Molecule details ›
Chain: A
Length: 205 amino acids
Theoretical weight: 22.85 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q6P4G7 (Residues: 5-213; Coverage: 79%)
Gene name: TRA@
Sequence domains:
Structure domains: Immunoglobulins
T cell receptor beta constant 1 Chain: B
Molecule details ›
Chain: B
Length: 253 amino acids
Theoretical weight: 28.29 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: P01850 (Residues: 1-129; Coverage: 73%)
Gene name: TRBC1
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Beta-2-microglobulin; Major histocompatibility complex class I-related gene protein Chain: C
Molecule details ›
Chain: C
Length: 392 amino acids
Theoretical weight: 45.42 KDa
Source organism: Bos taurus
Expression system: Trichoplusia ni
UniProt:
  • Canonical: C1ITJ8 (Residues: 19-295; Coverage: 87%)
  • Canonical: P01888 (Residues: 21-118; Coverage: 100%)
Gene names: B2M, MR1
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand SO4 3 x SO4
1 modified residue:
Ligand KFP 1 x KFP

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 23-ID-D
Spacegroup: P212121
Unit cell:
a: 82.045Å b: 87.41Å c: 156.372Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.216 0.213 0.27
Expression systems:
  • Escherichia coli
  • Trichoplusia ni

Quick links

Citations

34 review citations

Antigen Recognition by MR1-Reactive T Cells; MAIT Cells, Metabolites, and Remaining Mysteries.
Corbett et al. (2020)
33 more

2 mentions without citation

The molecular basis for Mucosal-Associated Invariant T cell recognition of MR1 proteins.
López-Sagaseta et al. (2013)
1 more