X-ray diffraction
2.2Å resolution

Human 14-3-3 isoform zeta in complex with a diphoyphorylated C-RAF peptide and Cotylenin A


Function and Biology Details

Reaction catalysed:
ATP + a protein = ADP + a phosphoprotein
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
14-3-3 protein zeta/delta Chains: A, B
Molecule details ›
Chains: A, B
Length: 235 amino acids
Theoretical weight: 26.72 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
  • Canonical: P63104 (Residues: 1-230; Coverage: 94%)
Gene name: YWHAZ
Sequence domains: 14-3-3 protein
Structure domains: 14-3-3 domain
RAF proto-oncogene serine/threonine-protein kinase Chain: P
Molecule details ›
Chain: P
Length: 36 amino acids
Theoretical weight: 4.18 KDa
Source organism: Homo sapiens
Expression system: Not provided
  • Canonical: P04049 (Residues: 229-264; Coverage: 6%)
Gene names: RAF, RAF1

Ligands and Environments

2 bound ligands:
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X10SA
Spacegroup: P212121
Unit cell:
a: 72.28Å b: 103.4Å c: 112.58Å
α: 90° β: 90° γ: 90°
R R work R free
0.202 0.2 0.243
Expression systems:
  • Escherichia coli
  • Not provided