› 4iam

X-ray diffraction
1.99Å resolution

Function and Biology Details

Reaction catalysed: 
GTP = 3',5'-cyclic GMP + diphosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo hexamer
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-187123 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Heme NO-binding domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.36 KDa
Source organism: Nostoc sp. PCC 7120 = FACHB-418
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8YUQ7 (Residues: 1-182; Coverage: 96%)
Gene name: alr2278
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments


Cofactor: Ligand HEM 2 x HEM
1 bound ligand:
Ligand MLI 2 x MLI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-2
Spacegroup: P213
Unit cell:
a: 122.626Å b: 122.626Å c: 122.626Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.16 0.126 0.159
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

16 review citations

Thirty Years of Saying NO: Sources, Fate, Actions, and Misfortunes of the Endothelium-Derived Vasodilator Mediator.
Vanhoutte et al. (2016)
15 more

3 mentions without citation

Insights into BAY 60-2770 activation and S-nitrosylation-dependent desensitization of soluble guanylyl cyclase via crystal structures of homologous nostoc H-NOX domain complexes.
Kumar et al. (2013)
2 more