PDBe 4iah

X-ray diffraction
2.8Å resolution

Crystal Structure of BAY 60-2770 bound C139A H-NOX domain with S-nitrosylated conserved C122

Released:

Function and Biology Details

Reaction catalysed:
GTP = 3',5'-cyclic GMP + diphosphate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo trimer
homo hexamer
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Alr2278 protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 182 amino acids
Theoretical weight: 20.39 KDa
Source organism: Nostoc sp. PCC 7120
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8YUQ7 (Residues: 1-182; Coverage: 96%)
Gene name: alr2278
Sequence domains: Haem-NO-binding
Structure domains: H-NOX domain

Ligands and Environments

2 bound ligands:

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU MICROMAX-007 HF
Spacegroup: P213
Unit cell:
a: 122.573Å b: 122.573Å c: 122.573Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.166 0.163 0.218
Expression system: Escherichia coli BL21(DE3)