PDBe 4i5i

X-ray diffraction
2.5Å resolution

Crystal structure of the SIRT1 catalytic domain bound to NAD and an EX527 analog


Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
SirtT1 75 kDa fragment Chains: A, B
Molecule details ›
Chains: A, B
Length: 287 amino acids
Theoretical weight: 32.52 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
  • Canonical: Q96EB6 (Residues: 241-516; Coverage: 37%)
Gene names: SIR2L1, SIRT1
Sequence domains: Sir2 family
Structure domains:

Ligands and Environments

3 bound ligands:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P32
Unit cell:
a: 106.513Å b: 106.513Å c: 80.663Å
α: 90° β: 90° γ: 120°
R R work R free
0.169 0.167 0.195
Expression system: Spodoptera frugiperda