4i0b

X-ray diffraction
1.5Å resolution

structure of the mutant Catabolite gene activator protein H160L

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
cAMP-activated global transcriptional regulator CRP Chains: A, B
Molecule details ›
Chains: A, B
Length: 222 amino acids
Theoretical weight: 25.05 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli
UniProt:
  • Canonical: P0ACJ8 (Residues: 1-210; Coverage: 100%)
Gene names: JW5702, b3357, cap, crp, csm
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P21
Unit cell:
a: 56.78Å b: 72.32Å c: 58.41Å
α: 90° β: 109.89° γ: 90°
R-values:
R R work R free
0.182 0.18 0.208
Expression system: Escherichia coli