4hut

X-ray diffraction
1.95Å resolution

Structure of ATP:co(I)rrinoid adenosyltransferase (CobA) from Salmonella enterica in complex with four and five-coordinate cob(II)alamin and ATP

Released:

Function and Biology Details

Reaction catalysed:
(1a) 2 cob(II)alamin + 2 [corrinoid adenosyltransferase] = 2 [corrinoid adenosyltransferase]-cob(II)alamin
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Corrinoid adenosyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 191 amino acids
Theoretical weight: 21.13 KDa
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium str. LT2
Expression system: Salmonella enterica subsp. enterica serovar Typhimurium
UniProt:
  • Canonical: P31570 (Residues: 6-196; Coverage: 97%)
Gene names: STM1718, btuR, cobA
Sequence domains: ATP:corrinoid adenosyltransferase BtuR/CobO/CobP
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 59.862Å b: 74.223Å c: 92.396Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.175 0.232
Expression system: Salmonella enterica subsp. enterica serovar Typhimurium