4hp3

X-ray diffraction
2.05Å resolution

Function and Biology Details

Reaction catalysed:
DNA 5-methylcytosine + 2-oxoglutarate + O(2) = DNA 5-hydroxymethylcytosine + succinate + CO(2) 
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
1 distinct DNA molecule
Macromolecules (2 distinct):
Methylcytosine dioxygenase tet3 Chain: C
Molecule details ›
Chain: C
Length: 72 amino acids
Theoretical weight: 8.43 KDa
Source organism: Xenopus tropicalis
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A0JP82 (Residues: 58-111; Coverage: 3%)
Gene name: tet3
DNA (5'-D(*GP*CP*CP*AP*AP*CP*GP*TP*TP*GP*GP*C)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 12 nucleotides
Theoretical weight: 3.66 KDa
Source organism: Xenopus tropicalis
Expression system: Not provided

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: C2
Unit cell:
a: 69.892Å b: 39.455Å c: 54.134Å
α: 90° β: 99.92° γ: 90°
R-values:
R R work R free
0.217 0.216 0.243
Expression systems:
  • Escherichia coli BL21
  • Not provided