PDBe 4hkj

X-ray diffraction
3Å resolution

Structure of Cowpox CPXV203 in complex with MHCI (H-2Kb)

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
H-2 class I histocompatibility antigen, K-B alpha chain Chains: A, E, I, M
Molecule details ›
Chains: A, E, I, M
Length: 280 amino acids
Theoretical weight: 32.26 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P01901 (Residues: 22-301; Coverage: 81%)
Gene names: H2-K, H2-K1
Sequence domains:
Structure domains:
Beta-2-microglobulin Chains: B, F, J, N
Molecule details ›
Chains: B, F, J, N
Length: 100 amino acids
Theoretical weight: 11.88 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P61769 (Residues: 21-119; Coverage: 100%)
Gene names: B2M, CDABP0092, HDCMA22P
Sequence domains: Immunoglobulin C1-set domain
Structure domains: Immunoglobulins
Ovalbumin Chains: C, G, K, O
Molecule details ›
Chains: C, G, K, O
Length: 8 amino acids
Theoretical weight: 964 Da
Source organism: Gallus gallus
Expression system: Not provided
UniProt:
  • Canonical: P01012 (Residues: 258-265; Coverage: 2%)
Gene name: SERPINB14
CPXV203 protein Chains: D, H, L, P
Molecule details ›
Chains: D, H, L, P
Length: 206 amino acids
Theoretical weight: 23.9 KDa
Source organism: Cowpox virus (Brighton Red)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8QMP2 (Residues: 17-221; Coverage: 91%)
Gene name: CPXV203 CDS
Sequence domains: Poxvirus T4 protein, N terminus
Structure domains: Viral Chemokine Inhibitor; Chain A

Ligands and Environments

No bound ligands

1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 4.2.2
Spacegroup: P1
Unit cell:
a: 88.31Å b: 88.25Å c: 106.42Å
α: 76.18° β: 69.29° γ: 66.69°
R-values:
R R work R free
0.231 0.23 0.253
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided