4hi0

X-ray diffraction
2.35Å resolution

Crystal Structure of Helicobacter pylori Urease Accessory Protein UreF/H/G complex

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
Urease accessory protein UreF Chains: A, C
Molecule details ›
Chains: A, C
Length: 254 amino acids
Theoretical weight: 28.65 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09065 (Residues: 1-254; Coverage: 100%)
Gene names: HP_0069, ureF
Sequence domains: UreF
Structure domains: Urease accessory protein UreF
Urease accessory protein UreH Chains: B, D
Molecule details ›
Chains: B, D
Length: 265 amino acids
Theoretical weight: 29.76 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09067 (Residues: 1-265; Coverage: 100%)
Gene names: HP_0067, ureH
Sequence domains: UreD urease accessory protein
Urease accessory protein UreG Chains: E, F
Molecule details ›
Chains: E, F
Length: 199 amino acids
Theoretical weight: 21.98 KDa
Source organism: Helicobacter pylori 26695
Expression system: Escherichia coli
UniProt:
  • Canonical: Q09066 (Residues: 1-199; Coverage: 100%)
Gene names: HP_0068, ureG
Sequence domains: CobW/HypB/UreG, nucleotide-binding domain
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: DIAMOND BEAMLINE I04
Spacegroup: P212121
Unit cell:
a: 76.124Å b: 96.429Å c: 237.452Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.193 0.192 0.242
Expression system: Escherichia coli