4hca

X-ray diffraction
2.8Å resolution

DNA binding by GATA transcription factor-complex 1

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Trans-acting T-cell-specific transcription factor GATA-3 Chain: A
Molecule details ›
Chain: A
Length: 115 amino acids
Theoretical weight: 12.89 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P23771 (Residues: 260-370; Coverage: 25%)
Gene name: GATA3
Sequence domains: GATA zinc finger
Structure domains: Erythroid Transcription Factor GATA-1, subunit A
DNA (5'-D(*AP*AP*TP*GP*TP*CP*CP*AP*TP*CP*TP*GP*AP*TP*AP*AP*GP*AP*CP*G)-3') Chain: X
Molecule details ›
Chain: X
Length: 20 nucleotides
Theoretical weight: 6.14 KDa
Source organism: Homo sapiens
Expression system: Not provided
DNA (5'-D(*TP*TP*CP*GP*TP*CP*TP*TP*AP*TP*CP*AP*GP*AP*TP*GP*GP*AP*CP*A)-3') Chain: Y
Molecule details ›
Chain: Y
Length: 20 nucleotides
Theoretical weight: 6.12 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P21
Unit cell:
a: 54.486Å b: 36.55Å c: 66.118Å
α: 90° β: 103.7° γ: 90°
R-values:
R R work R free
0.247 0.245 0.296
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided