PDBe 4h83

X-ray diffraction
2.09Å resolution

Crystal structure of Mandelate racemase/muconate lactonizing enzyme (EFI target:502127)

Entry authors: Kim J, Toro R, Bhosle R, Al Obaidi NF, Morisco LL, Wasserman SR, Sojitra S, Washington E, Scott Glenn A, Chowdhury S, Evans B, Hammonds J, Stead M, Hillerich B, Love J, Seidel RD, Imker HJ, Gerlt JA, Almo SC, Enzyme Function Initiative (EFI)

Function and Biology Details

Structure analysis Details

Assemblies composition:
homo hexamer (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
MR_MLE domain-containing protein Chains: A, B, C, D, E, F
Molecule details ›
Chains: A, B, C, D, E, F
Length: 388 amino acids
Theoretical weight: 43.49 KDa
Source organism: marine actinobacterium PHSC20C1
Expression system: Escherichia coli
  • Canonical: A4AFX2 (Residues: 1-388; Coverage: 100%)
Gene name: A20C1_08358
Sequence domains:
Structure domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P212121
Unit cell:
a: 89.983Å b: 102.95Å c: 234.431Å
α: 90° β: 90° γ: 90°
R R work R free
0.15 0.147 0.204
Expression system: Escherichia coli