4h6d

X-ray diffraction
2.9Å resolution

Crystal structure of PLP-soaked HMP synthase Thi5 from S. cerevisiae

Released:

Function and Biology Details

Biochemical function:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
4-amino-5-hydroxymethyl-2-methylpyrimidine phosphate synthase THI5 Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 346 amino acids
Theoretical weight: 39.42 KDa
Source organism: Saccharomyces cerevisiae
Expression system: Escherichia coli
UniProt:
  • Canonical: P43534 (Residues: 1-340; Coverage: 100%)
Gene names: MOL2, THI5, YFL058W
Sequence domains: NMT1/THI5 like
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID14-4
Spacegroup: P21
Unit cell:
a: 79.48Å b: 191.84Å c: 101.89Å
α: 90° β: 112.72° γ: 90°
R-values:
R R work R free
0.223 0.221 0.263
Expression system: Escherichia coli