4guu

X-ray diffraction
2.3Å resolution

Function and Biology Details

Reaction catalysed:
(1a) a [histone H3]-N(6),N(6)-dimethyl-L-lysine(4) + acceptor + H(2)O = a [histone H3]-N(6)-methyl-L-lysine(4) + formaldehyde + reduced acceptor
Biochemical function:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Lysine-specific histone demethylase 1B Chain: A
Molecule details ›
Chain: A
Length: 776 amino acids
Theoretical weight: 87.05 KDa
Source organism: Homo sapiens
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: Q8NB78 (Residues: 51-822; Coverage: 94%)
Gene names: AOF1, C6orf193, KDM1B, LSD2
Sequence domains:
Putative oxidoreductase GLYR1 Chain: B
Molecule details ›
Chain: B
Length: 124 amino acids
Theoretical weight: 13.53 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q49A26 (Residues: 152-268; Coverage: 21%)
Gene names: GLYR1, HIBDL, NDF, NP60

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21
Unit cell:
a: 62.82Å b: 90.157Å c: 87.824Å
α: 90° β: 104.2° γ: 90°
R-values:
R R work R free
0.2 0.199 0.231
Expression systems:
  • Spodoptera frugiperda
  • Escherichia coli