4gk5

X-ray diffraction
3.21Å resolution

Crystal structure of human Rev3-Rev7-Rev1-Polkappa complex

Released:
Source organism: Homo sapiens
Primary publication:
Structural insights into the assembly of human translesion polymerase complexes.
Protein Cell 3 864-74 (2012)
PMID: 23143872

Function and Biology Details

Reaction catalysed:
Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1)

Structure analysis Details

Assemblies composition:
Non-polymer only tetramer (preferred)
Non-polymer only trimer
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
Mitotic spindle assembly checkpoint protein MAD2B Chains: A, B
Molecule details ›
Chains: A, B
Length: 238 amino acids
Theoretical weight: 26.96 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UI95 (Residues: 1-211; Coverage: 100%)
Gene names: MAD2B, MAD2L2, REV7
Sequence domains: HORMA domain
Structure domains: HORMA domain
DNA polymerase zeta catalytic subunit Chains: C, D
Molecule details ›
Chains: C, D
Length: 52 amino acids
Theoretical weight: 5.63 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: O60673 (Residues: 1847-1898; Coverage: 2%)
Gene names: POLZ, REV3, REV3L
DNA repair protein REV1 Chains: E, F
Molecule details ›
Chains: E, F
Length: 136 amino acids
Theoretical weight: 15.18 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9UBZ9 (Residues: 1117-1251; Coverage: 11%)
Gene names: REV1, REV1L
Sequence domains: DNA repair protein REV1 C-terminal domain
Structure domains: Methane Monooxygenase Hydroxylase; Chain G, domain 1
DNA polymerase kappa Chain: G
Molecule details ›
Chain: G
Length: 10 amino acids
Theoretical weight: 1.28 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: Q9UBT6 (Residues: 564-573; Coverage: 1%)
Gene names: DINB1, POLK

Ligands and Environments

No bound ligands

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRF BEAMLINE BL17U
Spacegroup: P21212
Unit cell:
a: 119.998Å b: 72.762Å c: 104.653Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.234 0.232 0.281
Expression systems:
  • Escherichia coli
  • Not provided