4g92

X-ray diffraction
1.8Å resolution

CCAAT-binding complex from Aspergillus nidulans with DNA

Released:

Function and Biology Details

Structure analysis Details

Assembly composition:
hetero pentamer (preferred)
Entry contents:
3 distinct polypeptide molecules
2 distinct DNA molecules
Macromolecules (5 distinct):
HAPB protein Chain: A
Molecule details ›
Chain: A
Length: 64 amino acids
Theoretical weight: 7.67 KDa
Source organism: Aspergillus nidulans
Expression system: Escherichia coli
UniProt:
  • Canonical: P87249 (Residues: 231-293; Coverage: 17%)
Gene name: hapB
Sequence domains: CCAAT-binding transcription factor (CBF-B/NF-YA) subunit B
CBFD_NFYB_HMF domain-containing protein Chain: B
Molecule details ›
Chain: B
Length: 92 amino acids
Theoretical weight: 10.64 KDa
Source organism: Aspergillus nidulans FGSC A4
Expression system: Escherichia coli
UniProt:
  • Canonical: Q5B5Z6 (Residues: 41-132; Coverage: 42%)
Gene name: ANIA_04034
Sequence domains: Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Structure domains: Histone, subunit A
CBFD_NFYB_HMF domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 119 amino acids
Theoretical weight: 13.67 KDa
Source organism: Aspergillus nidulans FGSC A4
Expression system: Escherichia coli
UniProt:
  • Canonical: C8V0B5 (Residues: 47-164; Coverage: 45%)
Gene name: ANIA_06492
Sequence domains: Histone-like transcription factor (CBF/NF-Y) and archaeal histone
Structure domains: Histone, subunit A
DNA Chain: D
Molecule details ›
Chain: D
Length: 25 nucleotides
Theoretical weight: 7.57 KDa
DNA Chain: E
Molecule details ›
Chain: E
Length: 25 nucleotides
Theoretical weight: 7.78 KDa

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SLS BEAMLINE X06SA
Spacegroup: P212121
Unit cell:
a: 57.04Å b: 72.89Å c: 145.89Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.158 0.157 0.19
Expression system: Escherichia coli