PDBe 4g0b

X-ray diffraction
3Å resolution

Structure of native HCT from Coffea canephora


Function and Biology Details

Reaction catalysed:
4-coumaroyl-CoA + shikimate = CoA + 4-coumaroylshikimate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Entry contents:
1 distinct polypeptide molecule
Hydroxycinnamoyl-CoA shikimate/quinate hydroxycinnamoyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 436 amino acids
Theoretical weight: 48.24 KDa
Source organism: Coffea canephora
Expression system: Escherichia coli BL21(DE3)
  • Canonical: A4ZKE4 (Residues: 1-434; Coverage: 100%)
Gene name: HCT
Sequence domains: Transferase family
Structure domains: Chloramphenicol acetyltransferase-like domain

Ligands and Environments

1 bound ligand:

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P42212
Unit cell:
a: 116.1Å b: 116.1Å c: 158.91Å
α: 90° β: 90° γ: 90°
R R work R free
0.189 0.186 0.25
Expression system: Escherichia coli BL21(DE3)