4fzw

X-ray diffraction
2.55Å resolution

Crystal Structure of the PaaF-PaaG Hydratase-Isomerase Complex from E.coli

Released:

Function and Biology Details

Reactions catalysed:
(3S)-3-hydroxyacyl-CoA = trans-2(or 3)-enoyl-CoA + H(2)O
2-(1,2-epoxy-1,2-dihydrophenyl)acetyl-CoA = 2-oxepin-2(3H)-ylideneacetyl-CoA
Biochemical function:
Biological process:
Cellular component:

Structure analysis Details

Assembly composition:
hetero dodecamer (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
2,3-dehydroadipyl-CoA hydratase Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 27.64 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P76082 (Residues: 1-255; Coverage: 100%)
Gene names: JW1388, b1393, paaF, ydbR
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:
1,2-epoxyphenylacetyl-CoA isomerase Chains: C, D
Molecule details ›
Chains: C, D
Length: 274 amino acids
Theoretical weight: 29.77 KDa
Source organism: Escherichia coli K-12
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P77467 (Residues: 1-262; Coverage: 100%)
Gene names: JW1389, b1394, paaG, ydbT
Sequence domains: Enoyl-CoA hydratase/isomerase
Structure domains:

Ligands and Environments

1 bound ligand:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P321
Unit cell:
a: 131.976Å b: 131.976Å c: 153.865Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.282 0.28 0.321
Expression system: Escherichia coli BL21(DE3)