4fxp

X-ray diffraction
1.95Å resolution

Crystal structure of adenosine 5'-phosphosulfate kinase from Arabidopsis thaliana in Complex with Sulfate and APS

Released:
Source organism: Arabidopsis thaliana

Function and Biology Details

Reaction catalysed:
ATP + adenylyl sulfate = ADP + 3'-phosphoadenylyl sulfate
Biochemical function:
Biological process:
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Adenylyl-sulfate kinase 1, chloroplastic Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 200 amino acids
Theoretical weight: 22.1 KDa
Source organism: Arabidopsis thaliana
Expression system: Escherichia coli
UniProt:
  • Canonical: Q43295 (Residues: 77-276; Coverage: 73%)
Gene names: AKN1, APK1, At2g14750, F26C24.11, T6B13.1
Sequence domains: Adenylylsulphate kinase
Structure domains: P-loop containing nucleotide triphosphate hydrolases

Ligands and Environments

2 bound ligands:
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: C2
Unit cell:
a: 120.948Å b: 92.348Å c: 73.183Å
α: 90° β: 113.52° γ: 90°
R-values:
R R work R free
0.167 0.166 0.189
Expression system: Escherichia coli