4fwj

X-ray diffraction
2.9Å resolution

Native structure of LSD2/AOF1/KDM1b in spacegroup of I222 at 2.9A

Released:

Function and Biology Details

Reaction catalysed:
(1a) a [histone H3]-N(6),N(6)-dimethyl-L-lysine(4) + acceptor + H(2)O = a [histone H3]-N(6)-methyl-L-lysine(4) + formaldehyde + reduced acceptor
Biochemical function:
Cellular component:

Structure analysis Details

Assemblies composition:
monomeric
homo dimer (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Lysine-specific histone demethylase 1B Chains: A, B
Molecule details ›
Chains: A, B
Length: 796 amino acids
Theoretical weight: 89.34 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8NB78 (Residues: 30-822; Coverage: 97%)
Gene names: AOF1, C6orf193, KDM1B, LSD2
Sequence domains:

Ligands and Environments

No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BSRF BEAMLINE 1W2B
Spacegroup: I222
Unit cell:
a: 144.322Å b: 170.933Å c: 202.566Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.188 0.186 0.222
Expression system: Escherichia coli